|Sample Requirement||Turnaround Time|
Gelatin-coated slide and fixed
Same working day
Endometrial smears are simple and quick to perform and provide a much more accurate and direct test for the diagnosis of acute endometritis in mares, pre-coitus than with swab examinations alone. When used in conjunction with endometrial swabs for bacteriological examinations, results are infinitely more meaningful, interpretable and therefore valuable. The presence of endometrial epithelial cells is used as a test of smear quality and the presence or absence of polymorphonuclear leucocytes is used as the diagnostic test for acute endometritis.
Smears may be collected during oestrus by a variety of methods, but we favour a simple non-guarded technique. An extended, sterile, large tipped swab is passed via a sterile speculum, through the relaxed cervix and rotated onto the endometrial lining. The swab is withdrawn and should be rolled onto gelatine-coated slides immediately and fixed with 'Smear- fix', carbowax or even hair spray prior to transport to the laboratory for staining.
Excellent results have been obtained by rolling smears onto 'testsimplets' (Boehringer Mannheim UK Ltd.) pre-stained slides, then after two to three minutes at room temperature, washing off the background blue colour, drying and cover slipping. This technique provides an excellent permanent smear sample for immediate reading and/or for sending for a second opinion.